Kit package includes lightcycler 480 thermal block unit 96 or 384well, block cover, storage box. Roche lightcycler 480 ii inquire about availability includes computer loaded with lightcycler 480 software release 1. It compares each individual melting curve profile to a standard, and then makes a call. Roche life science realtime ready overview lightcycler. The complete security suite for all your datacommunication needs invisible secrets encryption software 4 not only encrypts your data and files for safe keeping or for secure transfer across the net, it also hides them in places that on the surface appear totally innocent, such as picture or sound files, or web pages. See your instruments user manual for detailed instructions on how to configure the run method. For gene expression and gene dosage studies the highly flexible lightcycler 480 relative quantification software provides the user with more than one choice for relative quantification. The roche lightcycler 480 rtpcr system combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, geneknockdown studies, and snp.
High resolution melting hrm is a novel, homogeneous, closetube, postpcr method, enabling genomic researchers to analyze genetic variations snps, mutations, methylations in pcr amplicons. For simultaneous analysis, all primer pairs need to be amplified under identical conditions, preferably on one plate with a layout that is straightforward and convenient. The lightcycler 480 system is a highperformance, medium to highthroughput pcr platform 96 or 384well plates that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Identify more new variants, quickly and accurately.
This compact benchtop instrument uses a fully integrated multi well plate that can be switched out for either 96. Roche lightcycler 480 realtime pcr machine bosch bosch. The hardware, software, and high resolution melting master containing a proprietary dnasaturating dye are optimally designed to work together to support this powerful method. All the samples with a late amplification, as monitored by realtime pcr or associated with fluorescence of less than the 60% of the maximum, were excluded from the analysis. Absolute and relative quantification analysis methods, and subtypes of these techniques are implemented in the lightcycler 480 software. Owing to its superior temperature homogeneity, flexible detection channels and powerful software analysis modules, the lightcycler 480 system fulfills the requirements of both basic and advanced genotyping methods. Lightcycler realtime pcr systems application manual.
Additional innovative lightcycler 480 software modules e. Lightcycler 480 realtime pcr system from roche applied. To determine genotypes, the software analyzes the shapes of all the melting curves. Roche lightcycler 480 real time pcr the lab world group. The lightcycler 480 gene scanning software scans pcr fragments for the presence of genetic variations e. Lightcycler 480 realtime pcr system rapid by nature. The lightcycler 480 gene scanning software can differentiating homozygous samples when the amplicons from heterozygous samples containing both homo and heteroduplexes are melted, the resulting curves have a characteristic shape, different from the typical shape that results from a homozygous sample.
By offering a high resolution melting master pcr reagent and a special gene scanning analysis software for the lightcycler 480 system. The lightcycler 480 ii is a realtime pcr cycler for mid to high sample throughput. Based on unique lightcycler algorithms, the sophisticated lightcycler 480. The software is compatible with the regulations for electronic records subpart b. Lightcycler 480 multiple plate analysis software 05 075 122 001. The new lightcycler 480 system includes sophisti cated software algorithms that generate data for absolute and relative quantification. The lightcycler 480 gene scanning software is intended for general laboratory use in combination with the lightcycler 480 instrument and lightcycler 480 software. It goes beyond the power of classical melting curve analysis by allowing to study the thermal denaturation of a doublestranded dna in much more detail and with much higher information yield than ever before.
Roche applied science lightcycler 480 realtime pcr system. Roche lightcycler 480 ii96 pcr thermocycler 96silber block cycler cover dell latitude i5 running windows 10 with lightcycler 480 software ver1. Contact your roche representative for further information. I prepared the twostep cycling experiment with the following conditions. The roche lightcycler 480 realtime pcr system offers realtime pcr analysis of gene expression, genetic variation and melting curvebased mutation. The modern instrument design, outstanding technical and software features, as well. Lightcycler software 4, free lightcycler software 4 software downloads. V license statements for the lightcycler 480 gene scanning software parts of the software used for the lightcycler 480 system are licensed from idaho. The lightcycler 480 software enables users to quickly customize the system to meet different application requirements, from basic manual analysis to automated evaluation of data using the software macro feature. Lightcycler 480 realtime pcr system high resolution melting. Used roche lightcycler roche lightcycler 480ii96 pcr. The lightcycler 480 realtime pcr system roche applied science brings you one more breakthrough in the realtime pcr analysis of gene expression and genetic variation roche applied sciences cuttingedge gene analysis technology is now available in a highthroughput, microwell platebased cycler format 96 or 384well.
The lightcycler 480 system offers high resolution meltingbased gene scanning as an integrated solution on a platebased realtime pcr instrument. Following the above outline, the genes of interest 1. Learn more about high resolution melting and gene scanning with the lightcycler 480 system. Support all current realtime pcr formats and applications gene expression, snp analysis, haplotyping, mutation scanning lightcycler 480 software version 1. Taqman fast advanced master mix on the roche lightcycler 480. Roche diagnostics lightcycler 480 probes master lightcycler. It compares each individual melting curve profile to. Realtime ready assays are available as single assays for the selected target or preplated on lightcycler 480 multiwell plates 96 or 384. Used roche lightcycler roche lightcycler 480ii96 pcr unit 3.
Lightcycler 480 software processes the raw melting curve data to form a difference plot. Start the lightcycler 480 software by doubleclicking the icon. It facilitates highly versatile and extensive data analysis to study gene expression and genetic variation. Lightcycler 480 system high resolution melting and gene.
Warranty information on warranty conditions are specified in the sales contract. Roche lightcycler 480ii96 pcr thermocycler 96silber block cycler cover dell latitude i5 running windows 10 with lightcycler 480 software ver1. The melting curves were normalized for direct comparison among samples. Realtime ready custom panels create your specific gene expression panel with realtime ready assays of your choice on lightcycler 480 multiwell plates 96 or 384, using the realtime ready configurator. The lightcycler 480 ii with led is suitable for detection systems with different probe types such as hybridization probes or hydrolysis 5nuclease taqman probes. Enter the username and assigned password to log in to the lightcycler 480 software. The gene scanning software module available for the lightcycler 480 software normalizes the raw melting curve data to form a difference plot. Any unauthorized modification of the instrument entails the invalidity of the guarantee. The lightcycler 480 system unleash the potential of realtime pcr. Amplify your success in medium and highthroughput realtime pcr applications.
The roche lightcycler 480 rtpcr system combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene knockdown studies, and snp analysis. The most important high resolution melting application is gene scanning the search for the. Lightcycler 480 sybr green i master version june 2005 easytouse hotstart reaction mix for pcr using the lightcycler 480 system cat. Furthermore, the lightcycler 480 software, version 1. The lightcycler 480 realtime pcr system can detect this dye, but it will not be used for fluorescence normalization in the lightcycler 480 system software.
The lightcycler 480 software provides innovative solutions for various quantitative realtime pcr qpcr analyses. Hrm curves were generated using the lightcycler 480 gene scanning software module with manual settings for sensitivity to 0. Roche molecular biochemicals lightcycler operators manual. Lightcycler 480 software for genetic variation analysis 58 lightcycler 480 multiple plate analysis software 59 221020908 realtime pcr application db 41020908 realtime pcr application db 4 006. Turn on the computer linked to the instrument and log on to window xp. Taqman fast advanced master mix on the roche lightcycler. With the accessory software module gene scanning analyze genetic variations snps. The lightcycler 480 basic software configuration includes.
Variant rs2237892 of kcnq1 is potentially associated with. Following the above outline, the gene s of interest 1. The roche lightcycler 480 system another breakthrough in realtime pcr analysis of gene expression and melting curvebased mutation analysis. Rely on the highly adaptable lightcycler 480 system and related software, kits, disposables, reagents, and probes to perform gene expression analysis, snp genotyping, and mutation scanning via high resolution melting hrm. The lightcycler 480 realtime pcr system is a versatile platform for the study of gene expression regulation and genetic variation4,5. I have a problem with the analysis in lightcycler 480 software. Enter username and password when the login dialog box appears and click the button to proceed with the login. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications. Lightcycler 480 genotyping software groups samples with similar melting profiles together and identifies each group as a genotype. High resolution melting hrm analysis is an alternative to dhplc sequencing screening of new gene variants. The platebased lightcycler 480 system is a highly adaptable and versatile realtime pcr system for the analysis of gene expression and genetic variation. See your instruments user manual for detailed instructions on how to configure the. Hardware, software and dyecontaining high resolution melting master mix have been developed in a concerted manner and are optimally designed to work together to support this novel application. The lightcycler 480 system unleash the potential of real.
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